AP Biology Period 2 #WongNation

Devansh Taori (Period Two) Biology Advanced Placement pGLO Laboratory Report Introduction The ultimate objective of this lab was to perform a genetic transformation. It is well established that gene expression is controlled by DNA. Genes are usually expressed through protein synthesis, a process where mRNA reads a sequence of DNA (transcription), processes it (usually only in eukaryotic cells), and proceeds to be transformed into a polypeptide when tRNA matches its anticodon with the codons on the mRNA (translation). Thus, if DNA can be altered in some way, then the gene expression can be altered as well. In this particular lab, we wanted to see if we could inject a sequence of DNA that codes for Green Fluorescent Protein into E. Coli bacteria. Since the specific pGLO plasmid codes for resistance to ampicillin, while the green fluorescent protein is only expressed in the presence of the sugar arabinose (a molecule which prompts transcription of the gene), the presence of the plas

Devansh Taori (per. 2) Biology AP Introduction Evolution occurs in populations of organisms and involves variation in the population, heredity, and differential survival. One way to study evolution is to study how the frequency of alleles in a population changes from generation to generation. Important questions surrounding the topic of evolution involve what the inheritance patterns of alleles, not just from two parental organisms, but also in a population are. Exploration of how allele frequencies change in populations can help us with predicting what will happen to a population in the future.   Mathematical models and computer simulations are tools used to explore the complexity of biological systems that might otherwise be difficult or impossible to study. Several models can be applied to questions about evolution. In this experiment, we built a spreadsheet that models a hypothetical gene pool changes from one generation to the next. The model exolores parameters that affe